Fig. 3

Trichrome and H&E staining technique are used to evaluate presence and severity of inflammatory cells, collagen accumulation, neovascularization, and other relevant factors. The evaluation involved assigning scores ranging from 0 to 3 (0 indicating absence, 1 indicating mild, 2 indicating moderate, and 3 indicating marked). (A) Hematoxylin stains nucleic acids a purple/blue color. Eosin stains components of the extracellular matrix/cytoplasm a pink color. In this slide H&E stain showing marked (score 3) degree of adhesion factors in Side-to-End anastomosis. (B) Trichrome stain showing characteristic pattern of histopathological findings and it stains collagen blue. In this slide Trichrome stain showing marked (score 3) degree of adhesion factors in Side-to-End anastomosis