Fig. 4

Trichrome and H&E staining technique are used to evaluate presence and severity of inflammatory cells, collagen accumulation, neovascularization, and other relevant factors. The evaluation involved assigning scores ranging from 0 to 3 (0 indicating absence, 1 indicating mild, 2 indicating moderate, and 3 indicating marked). (A) Hematoxylin stains nucleic acids a purple/blue color. Eosin stains components of the extracellular matrix/cytoplasm a pink color. In this slide H&E stain showing mild (score 1) degree of adhesion factors in End-to-End anastomosis. (B) Trichrome stain showing characteristic pattern of histopathological findings and it stains collagen blue. In this slide Trichrome stain showing mild (score 1) degree of adhesion factors in End-to-End anastomosis. (C) In this slide H&E stain showing moderate (score 2) degree of adhesion factors in End-to-End anastomosis. (D) In this slide Trichrome stain showing moderate (score 2) degree of adhesion factors in End-to-End anastomosis